var bibbase_data = {"data":"<img src=\"//bibbase.org/img/ajax-loader.gif\" id=\"spinner\"\n  style=\"display: none;\" alt=\"Loading..\" />\n\n<div id=\"bibbase\">\n\n  <script type=\"text/javascript\">\n    var bibbase = {\n      params: {\"bib\":\"https://api.zotero.org/users/3476755/collections/NJGLI74A/items?key=ckhubDoO51X1cQZZ35NkGdxk&format=bibtex&limit=100\",\"jsonp\":\"1\",\"authorFirst\":\"1\",\"hidemenu\":\"true\",\"commas\":\"true\",\"sort\":\"author_short\",\"groupby\":\"\",\"filter\":\"authors:(Inaba|Shiba|稲葉|柴)\",\"host\":\"bibbase.org\"},\n      data: [{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve\",\"volume\":\"147\",\"issn\":\"1470-1626, 1741-7899\",\"url\":\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\",\"doi\":\"10.1530/REP-13-0418\",\"abstract\":\"Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam ( Ruditapes philippinarum ), Pacific oyster ( Crassostrea gigas ), and Japanese scallop ( Patinopecten yessoensis ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K + /H + exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K + concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K + or ≥2.5 mM 4-aminopyridine, suggesting K + efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca 2 + is required for Ca 2 + /calmodulin-dependent flagellar beating. Ca 2 + influx occurs via Ca 2 + channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca 2 + channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca 2 + rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca 2 + channel blocker. Extracellular Na + is also essential for sperm motility initiation via regulation of Na + /Ca 2 + exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K + , Ca 2 + , and Na + .\",\"number\":\"3\",\"urldate\":\"2021-07-27\",\"journal\":\"REPRODUCTION\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Alavi\"],\"firstnames\":[\"Sayyed\",\"Mohammad\",\"Hadi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Matsumura\"],\"firstnames\":[\"Natsuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Itoh\"],\"firstnames\":[\"Naoki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Takahashi\"],\"firstnames\":[\"Keisuke\",\"G\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Osada\"],\"firstnames\":[\"Makoto\"],\"suffixes\":[]}],\"month\":\"March\",\"year\":\"2014\",\"pages\":\"331–345\",\"bibtex\":\"@article{alavi_roles_2014,\\n\\ttitle = {Roles of extracellular ions and {pH} in 5-{HT}-induced sperm motility in marine bivalve},\\n\\tvolume = {147},\\n\\tissn = {1470-1626, 1741-7899},\\n\\turl = {https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml},\\n\\tdoi = {10.1530/REP-13-0418},\\n\\tabstract = {Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (\\n              Ruditapes\\n              philippinarum\\n              ), Pacific oyster (\\n              Crassostrea gigas\\n              ), and Japanese scallop (\\n              Patinopecten yessoensis\\n              ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K\\n              +\\n              /H\\n              +\\n              exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K\\n              +\\n              concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K\\n              +\\n              or ≥2.5 mM 4-aminopyridine, suggesting K\\n              +\\n              efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca\\n              2\\n              +\\n              is required for Ca\\n              2\\n              +\\n              /calmodulin-dependent flagellar beating. Ca\\n              2\\n              +\\n              influx occurs via Ca\\n              2\\n              +\\n              channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca\\n              2\\n              +\\n              channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca\\n              2\\n              +\\n              rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca\\n              2\\n              +\\n              channel blocker. Extracellular Na\\n              +\\n              is also essential for sperm motility initiation via regulation of Na\\n              +\\n              /Ca\\n              2\\n              +\\n              exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K\\n              +\\n              , Ca\\n              2\\n              +\\n              , and Na\\n              +\\n              .},\\n\\tnumber = {3},\\n\\turldate = {2021-07-27},\\n\\tjournal = {REPRODUCTION},\\n\\tauthor = {Alavi, Sayyed Mohammad Hadi and Matsumura, Natsuki and Shiba, Kogiku and Itoh, Naoki and Takahashi, Keisuke G and Inaba, Kazuo and Osada, Makoto},\\n\\tmonth = mar,\\n\\tyear = {2014},\\n\\tpages = {331--345},\\n}\\n\\n\",\"author_short\":[\"Alavi, S. M. H.\",\"Matsumura, N.\",\"Shiba, K.\",\"Itoh, N.\",\"Takahashi, K. G\",\"Inaba, K.\",\"Osada, M.\"],\"key\":\"alavi_roles_2014\",\"id\":\"alavi_roles_2014\",\"bibbaseid\":\"alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity\",\"volume\":\"40\",\"issn\":\"0920-1742, 1573-5168\",\"url\":\"http://link.springer.com/10.1007/s10695-014-9933-8\",\"doi\":\"10.1007/s10695-014-9933-8\",\"language\":\"en\",\"number\":\"5\",\"urldate\":\"2021-07-27\",\"journal\":\"Fish Physiology and Biochemistry\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Alavi\"],\"firstnames\":[\"Sayyed\",\"Mohammad\",\"Hadi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Postlerová-Maňásková\"],\"firstnames\":[\"Pavla\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hatef\"],\"firstnames\":[\"Azadeh\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pšenička\"],\"firstnames\":[\"Martin\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pěknicová\"],\"firstnames\":[\"Jana\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ciereszko\"],\"firstnames\":[\"Andrzej\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Linhart\"],\"firstnames\":[\"Otomar\"],\"suffixes\":[]}],\"month\":\"October\",\"year\":\"2014\",\"pages\":\"1393–1398\",\"bibtex\":\"@article{alavi_protease_2014,\\n\\ttitle = {Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity},\\n\\tvolume = {40},\\n\\tissn = {0920-1742, 1573-5168},\\n\\turl = {http://link.springer.com/10.1007/s10695-014-9933-8},\\n\\tdoi = {10.1007/s10695-014-9933-8},\\n\\tlanguage = {en},\\n\\tnumber = {5},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Fish Physiology and Biochemistry},\\n\\tauthor = {Alavi, Sayyed Mohammad Hadi and Postlerová-Maňásková, Pavla and Hatef, Azadeh and Pšenička, Martin and Pěknicová, Jana and Inaba, Kazuo and Ciereszko, Andrzej and Linhart, Otomar},\\n\\tmonth = oct,\\n\\tyear = {2014},\\n\\tpages = {1393--1398},\\n}\\n\\n\",\"author_short\":[\"Alavi, S. M. H.\",\"Postlerová-Maňásková, P.\",\"Hatef, A.\",\"Pšenička, M.\",\"Pěknicová, J.\",\"Inaba, K.\",\"Ciereszko, A.\",\"Linhart, O.\"],\"key\":\"alavi_protease_2014\",\"id\":\"alavi_protease_2014\",\"bibbaseid\":\"alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://link.springer.com/10.1007/s10695-014-9933-8\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (<i>Coturnix japonica</i>)\",\"volume\":\"147\",\"issn\":\"1470-1626, 1741-7899\",\"url\":\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\",\"doi\":\"10.1530/REP-13-0439\",\"abstract\":\"Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility in vitro and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of HSP70 mRNA in the UVJ increases before ovulation/oviposition. Gene-specific in situ hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization in vivo . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.\",\"number\":\"2\",\"urldate\":\"2021-07-27\",\"journal\":\"REPRODUCTION\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Hiyama\"],\"firstnames\":[\"Gen\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Matsuzaki\"],\"firstnames\":[\"Mei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mizushima\"],\"firstnames\":[\"Shusei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dohra\"],\"firstnames\":[\"Hideo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ikegami\"],\"firstnames\":[\"Keisuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yoshimura\"],\"firstnames\":[\"Takashi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasanami\"],\"firstnames\":[\"Tomohiro\"],\"suffixes\":[]}],\"month\":\"February\",\"year\":\"2014\",\"pages\":\"167–178\",\"bibtex\":\"@article{hiyama_sperm_2014,\\n\\ttitle = {Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in {Japanese} quail (\\\\textit{{Coturnix} japonica})},\\n\\tvolume = {147},\\n\\tissn = {1470-1626, 1741-7899},\\n\\turl = {https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml},\\n\\tdoi = {10.1530/REP-13-0439},\\n\\tabstract = {Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility\\n              in vitro\\n              and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of\\n              HSP70\\n              mRNA in the UVJ increases before ovulation/oviposition. Gene-specific\\n              in situ\\n              hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization\\n              in vivo\\n              . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.},\\n\\tnumber = {2},\\n\\turldate = {2021-07-27},\\n\\tjournal = {REPRODUCTION},\\n\\tauthor = {Hiyama, Gen and Matsuzaki, Mei and Mizushima, Shusei and Dohra, Hideo and Ikegami, Keisuke and Yoshimura, Takashi and Shiba, Kogiku and Inaba, Kazuo and Sasanami, Tomohiro},\\n\\tmonth = feb,\\n\\tyear = {2014},\\n\\tpages = {167--178},\\n}\\n\\n\",\"author_short\":[\"Hiyama, G.\",\"Matsuzaki, M.\",\"Mizushima, S.\",\"Dohra, H.\",\"Ikegami, K.\",\"Yoshimura, T.\",\"Shiba, K.\",\"Inaba, K.\",\"Sasanami, T.\"],\"key\":\"hiyama_sperm_2014\",\"id\":\"hiyama_sperm_2014\",\"bibbaseid\":\"hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"incollection\",\"type\":\"incollection\",\"address\":\"Tokyo\",\"title\":\"Structure, Function, and Phylogenetic Consideration of Calaxin\",\"isbn\":\"978-4-431-54588-0 978-4-431-54589-7\",\"url\":\"http://link.springer.com/10.1007/978-4-431-54589-7_5\",\"language\":\"en\",\"urldate\":\"2021-07-27\",\"booktitle\":\"Sexual Reproduction in Animals and Plants\",\"publisher\":\"Springer Japan\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mizuno\"],\"firstnames\":[\"Katsutoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]}],\"editor\":[{\"propositions\":[],\"lastnames\":[\"Sawada\"],\"firstnames\":[\"Hitoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inoue\"],\"firstnames\":[\"Naokazu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Iwano\"],\"firstnames\":[\"Megumi\"],\"suffixes\":[]}],\"year\":\"2014\",\"doi\":\"10.1007/978-4-431-54589-7_5\",\"pages\":\"49–57\",\"bibtex\":\"@incollection{sawada_structure_2014,\\n\\taddress = {Tokyo},\\n\\ttitle = {Structure, {Function}, and {Phylogenetic} {Consideration} of {Calaxin}},\\n\\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\\n\\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_5},\\n\\tlanguage = {en},\\n\\turldate = {2021-07-27},\\n\\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\\n\\tpublisher = {Springer Japan},\\n\\tauthor = {Inaba, Kazuo and Mizuno, Katsutoshi and Shiba, Kogiku},\\n\\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\\n\\tyear = {2014},\\n\\tdoi = {10.1007/978-4-431-54589-7_5},\\n\\tpages = {49--57},\\n}\\n\\n\",\"author_short\":[\"Inaba, K.\",\"Mizuno, K.\",\"Shiba, K.\"],\"editor_short\":[\"Sawada, H.\",\"Inoue, N.\",\"Iwano, M.\"],\"key\":\"sawada_structure_2014\",\"id\":\"sawada_structure_2014\",\"bibbaseid\":\"inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://link.springer.com/10.1007/978-4-431-54589-7_5\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"The birth of quail chicks after intracytoplasmic sperm injection\",\"volume\":\"141\",\"issn\":\"1477-9129, 0950-1991\",\"url\":\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\",\"doi\":\"10.1242/dev.111765\",\"abstract\":\"Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.\",\"language\":\"en\",\"number\":\"19\",\"urldate\":\"2021-07-27\",\"journal\":\"Development\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Mizushima\"],\"firstnames\":[\"Shusei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hiyama\"],\"firstnames\":[\"Gen\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dohra\"],\"firstnames\":[\"Hideo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ono\"],\"firstnames\":[\"Tamao\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shimada\"],\"firstnames\":[\"Kiyoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasanami\"],\"firstnames\":[\"Tomohiro\"],\"suffixes\":[]}],\"month\":\"October\",\"year\":\"2014\",\"pages\":\"3799–3806\",\"bibtex\":\"@article{mizushima_birth_2014,\\n\\ttitle = {The birth of quail chicks after intracytoplasmic sperm injection},\\n\\tvolume = {141},\\n\\tissn = {1477-9129, 0950-1991},\\n\\turl = {https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic},\\n\\tdoi = {10.1242/dev.111765},\\n\\tabstract = {Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.},\\n\\tlanguage = {en},\\n\\tnumber = {19},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Development},\\n\\tauthor = {Mizushima, Shusei and Hiyama, Gen and Shiba, Kogiku and Inaba, Kazuo and Dohra, Hideo and Ono, Tamao and Shimada, Kiyoshi and Sasanami, Tomohiro},\\n\\tmonth = oct,\\n\\tyear = {2014},\\n\\tpages = {3799--3806},\\n}\\n\\n\",\"author_short\":[\"Mizushima, S.\",\"Hiyama, G.\",\"Shiba, K.\",\"Inaba, K.\",\"Dohra, H.\",\"Ono, T.\",\"Shimada, K.\",\"Sasanami, T.\"],\"key\":\"mizushima_birth_2014\",\"id\":\"mizushima_birth_2014\",\"bibbaseid\":\"mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"downloads\":4,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in <i>Ciona</i> Sperm\",\"volume\":\"15\",\"issn\":\"1422-0067\",\"url\":\"http://www.mdpi.com/1422-0067/15/8/13192\",\"doi\":\"10.3390/ijms150813192\",\"language\":\"en\",\"number\":\"8\",\"urldate\":\"2021-07-27\",\"journal\":\"International Journal of Molecular Sciences\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"July\",\"year\":\"2014\",\"pages\":\"13192–13208\",\"bibtex\":\"@article{shiba_distinct_2014,\\n\\ttitle = {Distinct {Roles} of {Soluble} and {Transmembrane} {Adenylyl} {Cyclases} in the {Regulation} of {Flagellar} {Motility} in \\\\textit{{Ciona}} {Sperm}},\\n\\tvolume = {15},\\n\\tissn = {1422-0067},\\n\\turl = {http://www.mdpi.com/1422-0067/15/8/13192},\\n\\tdoi = {10.3390/ijms150813192},\\n\\tlanguage = {en},\\n\\tnumber = {8},\\n\\turldate = {2021-07-27},\\n\\tjournal = {International Journal of Molecular Sciences},\\n\\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\\n\\tmonth = jul,\\n\\tyear = {2014},\\n\\tpages = {13192--13208},\\n}\\n\\n\",\"author_short\":[\"Shiba, K.\",\"Inaba, K.\"],\"key\":\"shiba_distinct_2014\",\"id\":\"shiba_distinct_2014\",\"bibbaseid\":\"shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://www.mdpi.com/1422-0067/15/8/13192\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO$_{\\\\textrm{2}}$\",\"volume\":\"137\",\"issn\":\"09247963\",\"url\":\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\",\"doi\":\"10.1016/j.jmarsys.2014.04.013\",\"language\":\"en\",\"urldate\":\"2021-07-27\",\"journal\":\"Journal of Marine Systems\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sung\"],\"firstnames\":[\"Chan-Gyung\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kim\"],\"firstnames\":[\"Tae\",\"Won\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Park\"],\"firstnames\":[\"Young-Gyu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kang\"],\"firstnames\":[\"Seong-Gil\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Choi\"],\"firstnames\":[\"Tae\",\"Seob\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Moon\"],\"firstnames\":[\"Seong-Dae\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Litvin\"],\"firstnames\":[\"Steve\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lee\"],\"firstnames\":[\"Kyu-Tae\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lee\"],\"firstnames\":[\"Jung-Suk\"],\"suffixes\":[]}],\"month\":\"September\",\"year\":\"2014\",\"pages\":\"67–73\",\"bibtex\":\"@article{sung_species_2014,\\n\\ttitle = {Species and gamete-specific fertilization success of two sea urchins under near future levels of {pCO}$_{\\\\textrm{2}}$},\\n\\tvolume = {137},\\n\\tissn = {09247963},\\n\\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992},\\n\\tdoi = {10.1016/j.jmarsys.2014.04.013},\\n\\tlanguage = {en},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Journal of Marine Systems},\\n\\tauthor = {Sung, Chan-Gyung and Kim, Tae Won and Park, Young-Gyu and Kang, Seong-Gil and Inaba, Kazuo and Shiba, Kogiku and Choi, Tae Seob and Moon, Seong-Dae and Litvin, Steve and Lee, Kyu-Tae and Lee, Jung-Suk},\\n\\tmonth = sep,\\n\\tyear = {2014},\\n\\tpages = {67--73},\\n}\\n\\n\",\"author_short\":[\"Sung, C.\",\"Kim, T. W.\",\"Park, Y.\",\"Kang, S.\",\"Inaba, K.\",\"Shiba, K.\",\"Choi, T. S.\",\"Moon, S.\",\"Litvin, S.\",\"Lee, K.\",\"Lee, J.\"],\"key\":\"sung_species_2014\",\"id\":\"sung_species_2014\",\"bibbaseid\":\"sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Sperm Proteases that May Be Involved in the Initiation of Sperm Motility in the Newt, <i>Cynops pyrrhogaster</i>\",\"volume\":\"15\",\"issn\":\"1422-0067\",\"url\":\"http://www.mdpi.com/1422-0067/15/9/15210\",\"doi\":\"10.3390/ijms150915210\",\"language\":\"en\",\"number\":\"9\",\"urldate\":\"2021-07-27\",\"journal\":\"International Journal of Molecular Sciences\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Yokoe\"],\"firstnames\":[\"Misato\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sano\"],\"firstnames\":[\"Makoto\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shibata\"],\"firstnames\":[\"Honami\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shibata\"],\"firstnames\":[\"Daisuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Takayama-Watanabe\"],\"firstnames\":[\"Eriko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Watanabe\"],\"firstnames\":[\"Akihiko\"],\"suffixes\":[]}],\"month\":\"August\",\"year\":\"2014\",\"pages\":\"15210–15224\",\"bibtex\":\"@article{yokoe_sperm_2014,\\n\\ttitle = {Sperm {Proteases} that {May} {Be} {Involved} in the {Initiation} of {Sperm} {Motility} in the {Newt}, \\\\textit{{Cynops} pyrrhogaster}},\\n\\tvolume = {15},\\n\\tissn = {1422-0067},\\n\\turl = {http://www.mdpi.com/1422-0067/15/9/15210},\\n\\tdoi = {10.3390/ijms150915210},\\n\\tlanguage = {en},\\n\\tnumber = {9},\\n\\turldate = {2021-07-27},\\n\\tjournal = {International Journal of Molecular Sciences},\\n\\tauthor = {Yokoe, Misato and Sano, Makoto and Shibata, Honami and Shibata, Daisuke and Takayama-Watanabe, Eriko and Inaba, Kazuo and Watanabe, Akihiko},\\n\\tmonth = aug,\\n\\tyear = {2014},\\n\\tpages = {15210--15224},\\n}\\n\\n\",\"author_short\":[\"Yokoe, M.\",\"Sano, M.\",\"Shibata, H.\",\"Shibata, D.\",\"Takayama-Watanabe, E.\",\"Inaba, K.\",\"Watanabe, A.\"],\"key\":\"yokoe_sperm_2014\",\"id\":\"yokoe_sperm_2014\",\"bibbaseid\":\"yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://www.mdpi.com/1422-0067/15/9/15210\"},\"metadata\":{\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\"html\":\"\"}],\n      metadata: {\"owner\":\"inaba, k\",\"authorlinks\":{\"inaba, k\":\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\"}},\n      ownerID: \"g8dxLP9AX2DH4M29s\"\n    };\n  </script>\n\n  <script type=\"text/javascript\">\n  var site$;\n  if (typeof $ != 'undefined') {\n    console.log('existing jquery: storing and then restoring');\n    site$ = $;\n    $ = null;\n  }\n  </script>\n\n  <script src=\"https://ajax.googleapis.com/ajax/libs/jquery/2.2.4/jquery.min.js\">\n  </script>\n  <script type=\"text/javascript\">\n  if (site$) {\n    console.log('existing jquery: avoiding conflict and restoring');\n    bb$ = $.noConflict(true);\n    $ = site$;\n  } else {\n    bb$ = $;\n  }\n  </script>\n\n  <script src=\"//bibbase.org/js/bibbase.min.js\"\n          type=\"text/javascript\">\n  </script>\n\n  <script type=\"text/javascript\"\n          src=\"https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.1/MathJax.js?config=TeX-AMS-MML_HTMLorMML\">\n  </script>\n  <script type=\"text/x-mathjax-config\">\n    MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\\\(','\\\\)']]},\n                        skipTags: [\"body\"],\n                        processClass: \"bibbase_paper\"});\n  </script>\n\n  <style>\n  @media print {\n    .dontprint {\n        display: none !important;\n    }\n\n  .bibbase_group {\n    background-color: #E0E0E0;\n    font-style: italic;\n    font-size: larger;\n    text-shadow: 0px 0px 3px #FFFFFF;\n    border-radius: 4px 4px 4px 4px;\n    -moz-border-radius: 4px 4px 4px 4px;\n    -webkit-border-radius: 4px;\n    padding: 5px 40px 5px;\n    margin-top: 10px;\n    margin-bottom: 5px;\n    cursor: pointer;\n  }\n\n  .bibbase_paper {\n    margin-bottom: 5px;\n  }\n\n  }\n  </style>\n\n  \n  <div style=\"float: right; 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	title = {Roles of extracellular ions and {pH} in 5-{HT}-induced sperm motility in marine bivalve},
	volume = {147},
	issn = {1470-1626, 1741-7899},
	url = {https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml},
	doi = {10.1530/REP-13-0418},
	abstract = {Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (
              Ruditapes
              philippinarum
              ), Pacific oyster (
              Crassostrea gigas
              ), and Japanese scallop (
              Patinopecten yessoensis
              ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K
              +
              /H
              +
              exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K
              +
              concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K
              +
              or ≥2.5 mM 4-aminopyridine, suggesting K
              +
              efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca
              2
              +
              is required for Ca
              2
              +
              /calmodulin-dependent flagellar beating. Ca
              2
              +
              influx occurs via Ca
              2
              +
              channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca
              2
              +
              channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca
              2
              +
              rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca
              2
              +
              channel blocker. Extracellular Na
              +
              is also essential for sperm motility initiation via regulation of Na
              +
              /Ca
              2
              +
              exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K
              +
              , Ca
              2
              +
              , and Na
              +
              .},
	number = {3},
	urldate = {2021-07-27},
	journal = {REPRODUCTION},
	author = {Alavi, Sayyed Mohammad Hadi and Matsumura, Natsuki and Shiba, Kogiku and Itoh, Naoki and Takahashi, Keisuke G and Inaba, Kazuo and Osada, Makoto},
	month = mar,
	year = {2014},
	pages = {331--345},
}



@article{alavi_protease_2014,
	title = {Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity},
	volume = {40},
	issn = {0920-1742, 1573-5168},
	url = {http://link.springer.com/10.1007/s10695-014-9933-8},
	doi = {10.1007/s10695-014-9933-8},
	language = {en},
	number = {5},
	urldate = {2021-07-27},
	journal = {Fish Physiology and Biochemistry},
	author = {Alavi, Sayyed Mohammad Hadi and Postlerová-Maňásková, Pavla and Hatef, Azadeh and Pšenička, Martin and Pěknicová, Jana and Inaba, Kazuo and Ciereszko, Andrzej and Linhart, Otomar},
	month = oct,
	year = {2014},
	pages = {1393--1398},
}



@article{hiyama_sperm_2014,
	title = {Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in {Japanese} quail (\textit{{Coturnix} japonica})},
	volume = {147},
	issn = {1470-1626, 1741-7899},
	url = {https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml},
	doi = {10.1530/REP-13-0439},
	abstract = {Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility
              in vitro
              and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of
              HSP70
              mRNA in the UVJ increases before ovulation/oviposition. Gene-specific
              in situ
              hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization
              in vivo
              . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.},
	number = {2},
	urldate = {2021-07-27},
	journal = {REPRODUCTION},
	author = {Hiyama, Gen and Matsuzaki, Mei and Mizushima, Shusei and Dohra, Hideo and Ikegami, Keisuke and Yoshimura, Takashi and Shiba, Kogiku and Inaba, Kazuo and Sasanami, Tomohiro},
	month = feb,
	year = {2014},
	pages = {167--178},
}



@incollection{sawada_structure_2014,
	address = {Tokyo},
	title = {Structure, {Function}, and {Phylogenetic} {Consideration} of {Calaxin}},
	isbn = {978-4-431-54588-0 978-4-431-54589-7},
	url = {http://link.springer.com/10.1007/978-4-431-54589-7_5},
	language = {en},
	urldate = {2021-07-27},
	booktitle = {Sexual {Reproduction} in {Animals} and {Plants}},
	publisher = {Springer Japan},
	author = {Inaba, Kazuo and Mizuno, Katsutoshi and Shiba, Kogiku},
	editor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},
	year = {2014},
	doi = {10.1007/978-4-431-54589-7_5},
	pages = {49--57},
}



@article{mizushima_birth_2014,
	title = {The birth of quail chicks after intracytoplasmic sperm injection},
	volume = {141},
	issn = {1477-9129, 0950-1991},
	url = {https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic},
	doi = {10.1242/dev.111765},
	abstract = {Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.},
	language = {en},
	number = {19},
	urldate = {2021-07-27},
	journal = {Development},
	author = {Mizushima, Shusei and Hiyama, Gen and Shiba, Kogiku and Inaba, Kazuo and Dohra, Hideo and Ono, Tamao and Shimada, Kiyoshi and Sasanami, Tomohiro},
	month = oct,
	year = {2014},
	pages = {3799--3806},
}



@article{shiba_distinct_2014,
	title = {Distinct {Roles} of {Soluble} and {Transmembrane} {Adenylyl} {Cyclases} in the {Regulation} of {Flagellar} {Motility} in \textit{{Ciona}} {Sperm}},
	volume = {15},
	issn = {1422-0067},
	url = {http://www.mdpi.com/1422-0067/15/8/13192},
	doi = {10.3390/ijms150813192},
	language = {en},
	number = {8},
	urldate = {2021-07-27},
	journal = {International Journal of Molecular Sciences},
	author = {Shiba, Kogiku and Inaba, Kazuo},
	month = jul,
	year = {2014},
	pages = {13192--13208},
}



@article{sung_species_2014,
	title = {Species and gamete-specific fertilization success of two sea urchins under near future levels of {pCO}$_{\textrm{2}}$},
	volume = {137},
	issn = {09247963},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992},
	doi = {10.1016/j.jmarsys.2014.04.013},
	language = {en},
	urldate = {2021-07-27},
	journal = {Journal of Marine Systems},
	author = {Sung, Chan-Gyung and Kim, Tae Won and Park, Young-Gyu and Kang, Seong-Gil and Inaba, Kazuo and Shiba, Kogiku and Choi, Tae Seob and Moon, Seong-Dae and Litvin, Steve and Lee, Kyu-Tae and Lee, Jung-Suk},
	month = sep,
	year = {2014},
	pages = {67--73},
}



@article{yokoe_sperm_2014,
	title = {Sperm {Proteases} that {May} {Be} {Involved} in the {Initiation} of {Sperm} {Motility} in the {Newt}, \textit{{Cynops} pyrrhogaster}},
	volume = {15},
	issn = {1422-0067},
	url = {http://www.mdpi.com/1422-0067/15/9/15210},
	doi = {10.3390/ijms150915210},
	language = {en},
	number = {9},
	urldate = {2021-07-27},
	journal = {International Journal of Molecular Sciences},
	author = {Yokoe, Misato and Sano, Makoto and Shibata, Honami and Shibata, Daisuke and Takayama-Watanabe, Eriko and Inaba, Kazuo and Watanabe, Akihiko},
	month = aug,
	year = {2014},
	pages = {15210--15224},
}

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G, <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>,  &amp; Osada, M.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml', event);\">\n    Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>REPRODUCTION</i>, 147(3): 331–345. March 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\"\n     onclick=\"log_download('alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1530/REP-13-0418\"\n     onclick=\"log_download('alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014', 'http://doi.org/10.1530/REP-13-0418', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{alavi_roles_2014,\n\ttitle = {Roles of extracellular ions and {pH} in 5-{HT}-induced sperm motility in marine bivalve},\n\tvolume = {147},\n\tissn = {1470-1626, 1741-7899},\n\turl = {https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml},\n\tdoi = {10.1530/REP-13-0418},\n\tabstract = {Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (\n              Ruditapes\n              philippinarum\n              ), Pacific oyster (\n              Crassostrea gigas\n              ), and Japanese scallop (\n              Patinopecten yessoensis\n              ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K\n              +\n              /H\n              +\n              exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K\n              +\n              concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K\n              +\n              or ≥2.5 mM 4-aminopyridine, suggesting K\n              +\n              efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca\n              2\n              +\n              is required for Ca\n              2\n              +\n              /calmodulin-dependent flagellar beating. Ca\n              2\n              +\n              influx occurs via Ca\n              2\n              +\n              channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca\n              2\n              +\n              channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca\n              2\n              +\n              rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca\n              2\n              +\n              channel blocker. Extracellular Na\n              +\n              is also essential for sperm motility initiation via regulation of Na\n              +\n              /Ca\n              2\n              +\n              exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K\n              +\n              , Ca\n              2\n              +\n              , and Na\n              +\n              .},\n\tnumber = {3},\n\turldate = {2021-07-27},\n\tjournal = {REPRODUCTION},\n\tauthor = {Alavi, Sayyed Mohammad Hadi and Matsumura, Natsuki and Shiba, Kogiku and Itoh, Naoki and Takahashi, Keisuke G and Inaba, Kazuo and Osada, Makoto},\n\tmonth = mar,\n\tyear = {2014},\n\tpages = {331--345},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam ( Ruditapes philippinarum ), Pacific oyster ( Crassostrea gigas ), and Japanese scallop ( Patinopecten yessoensis ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K + /H + exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K + concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K + or ≥2.5 mM 4-aminopyridine, suggesting K + efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca 2 + is required for Ca 2 + /calmodulin-dependent flagellar beating. Ca 2 + influx occurs via Ca 2 + channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca 2 + channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca 2 + rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca 2 + channel blocker. Extracellular Na + is also essential for sperm motility initiation via regulation of Na + /Ca 2 + exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K + , Ca 2 + , and Na + .\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Alavi, S. M. H., Postlerová-Maňásková, P., Hatef, A., Pšenička, M., Pěknicová, J., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>, Ciereszko, A.,  &amp; Linhart, O.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://link.springer.com/10.1007/s10695-014-9933-8\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014', 'http://link.springer.com/10.1007/s10695-014-9933-8', event);\">\n    Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Fish Physiology and Biochemistry</i>, 40(5): 1393–1398. October 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://link.springer.com/10.1007/s10695-014-9933-8\"\n     onclick=\"log_download('alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014', 'http://link.springer.com/10.1007/s10695-014-9933-8', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/s10695-014-9933-8\"\n     onclick=\"log_download('alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014', 'http://doi.org/10.1007/s10695-014-9933-8', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{alavi_protease_2014,\n\ttitle = {Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity},\n\tvolume = {40},\n\tissn = {0920-1742, 1573-5168},\n\turl = {http://link.springer.com/10.1007/s10695-014-9933-8},\n\tdoi = {10.1007/s10695-014-9933-8},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2021-07-27},\n\tjournal = {Fish Physiology and Biochemistry},\n\tauthor = {Alavi, Sayyed Mohammad Hadi and Postlerová-Maňásková, Pavla and Hatef, Azadeh and Pšenička, Martin and Pěknicová, Jana and Inaba, Kazuo and Ciereszko, Andrzej and Linhart, Otomar},\n\tmonth = oct,\n\tyear = {2014},\n\tpages = {1393--1398},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Hiyama, G., Matsuzaki, M., Mizushima, S., Dohra, H., Ikegami, K., Yoshimura, T., Shiba, K., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>,  &amp; Sasanami, T.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml', event);\">\n    Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (<i>Coturnix japonica</i>).\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>REPRODUCTION</i>, 147(2): 167–178. February 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\"\n     onclick=\"log_download('hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (&lt;i&gt;Coturnix japonica&lt;/i&gt;) [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1530/REP-13-0439\"\n     onclick=\"log_download('hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014', 'http://doi.org/10.1530/REP-13-0439', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{hiyama_sperm_2014,\n\ttitle = {Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in {Japanese} quail (\\textit{{Coturnix} japonica})},\n\tvolume = {147},\n\tissn = {1470-1626, 1741-7899},\n\turl = {https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml},\n\tdoi = {10.1530/REP-13-0439},\n\tabstract = {Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility\n              in vitro\n              and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of\n              HSP70\n              mRNA in the UVJ increases before ovulation/oviposition. Gene-specific\n              in situ\n              hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization\n              in vivo\n              . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.},\n\tnumber = {2},\n\turldate = {2021-07-27},\n\tjournal = {REPRODUCTION},\n\tauthor = {Hiyama, Gen and Matsuzaki, Mei and Mizushima, Shusei and Dohra, Hideo and Ikegami, Keisuke and Yoshimura, Takashi and Shiba, Kogiku and Inaba, Kazuo and Sasanami, Tomohiro},\n\tmonth = feb,\n\tyear = {2014},\n\tpages = {167--178},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility in vitro and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of HSP70 mRNA in the UVJ increases before ovulation/oviposition. Gene-specific in situ hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization in vivo . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>, Mizuno, K.,  &amp; Shiba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://link.springer.com/10.1007/978-4-431-54589-7_5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014', 'http://link.springer.com/10.1007/978-4-431-54589-7_5', event);\">\n    Structure, Function, and Phylogenetic Consideration of Calaxin.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  In Sawada, H., Inoue, N.,  &amp; Iwano, M., editor(s), <i>Sexual Reproduction in Animals and Plants</i>, pages 49–57. Springer Japan, Tokyo,  2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://link.springer.com/10.1007/978-4-431-54589-7_5\"\n     onclick=\"log_download('inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014', 'http://link.springer.com/10.1007/978-4-431-54589-7_5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Structure, Function, and Phylogenetic Consideration of Calaxin [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/978-4-431-54589-7_5\"\n     onclick=\"log_download('inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014', 'http://doi.org/10.1007/978-4-431-54589-7_5', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@incollection{sawada_structure_2014,\n\taddress = {Tokyo},\n\ttitle = {Structure, {Function}, and {Phylogenetic} {Consideration} of {Calaxin}},\n\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\n\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_5},\n\tlanguage = {en},\n\turldate = {2021-07-27},\n\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\n\tpublisher = {Springer Japan},\n\tauthor = {Inaba, Kazuo and Mizuno, Katsutoshi and Shiba, Kogiku},\n\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\n\tyear = {2014},\n\tdoi = {10.1007/978-4-431-54589-7_5},\n\tpages = {49--57},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Mizushima, S., Hiyama, G., Shiba, K., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>, Dohra, H., Ono, T., Shimada, K.,  &amp; Sasanami, T.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014', 'https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic', event);\">\n    The birth of quail chicks after intracytoplasmic sperm injection.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Development</i>, 141(19): 3799–3806. October 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\"\n     onclick=\"log_download('mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014', 'https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The birth of quail chicks after intracytoplasmic sperm injection [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1242/dev.111765\"\n     onclick=\"log_download('mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014', 'http://doi.org/10.1242/dev.111765', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>4 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{mizushima_birth_2014,\n\ttitle = {The birth of quail chicks after intracytoplasmic sperm injection},\n\tvolume = {141},\n\tissn = {1477-9129, 0950-1991},\n\turl = {https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic},\n\tdoi = {10.1242/dev.111765},\n\tabstract = {Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.},\n\tlanguage = {en},\n\tnumber = {19},\n\turldate = {2021-07-27},\n\tjournal = {Development},\n\tauthor = {Mizushima, Shusei and Hiyama, Gen and Shiba, Kogiku and Inaba, Kazuo and Dohra, Hideo and Ono, Tamao and Shimada, Kiyoshi and Sasanami, Tomohiro},\n\tmonth = oct,\n\tyear = {2014},\n\tpages = {3799--3806},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shiba, K.,  &amp; <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/8/13192\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014', 'http://www.mdpi.com/1422-0067/15/8/13192', event);\">\n    Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in <i>Ciona</i> Sperm.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>International Journal of Molecular Sciences</i>, 15(8): 13192–13208. July 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/8/13192\"\n     onclick=\"log_download('shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014', 'http://www.mdpi.com/1422-0067/15/8/13192', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in &lt;i&gt;Ciona&lt;/i&gt; Sperm [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/ijms150813192\"\n     onclick=\"log_download('shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014', 'http://doi.org/10.3390/ijms150813192', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shiba_distinct_2014,\n\ttitle = {Distinct {Roles} of {Soluble} and {Transmembrane} {Adenylyl} {Cyclases} in the {Regulation} of {Flagellar} {Motility} in \\textit{{Ciona}} {Sperm}},\n\tvolume = {15},\n\tissn = {1422-0067},\n\turl = {http://www.mdpi.com/1422-0067/15/8/13192},\n\tdoi = {10.3390/ijms150813192},\n\tlanguage = {en},\n\tnumber = {8},\n\turldate = {2021-07-27},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = jul,\n\tyear = {2014},\n\tpages = {13192--13208},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Sung, C., Kim, T. W., Park, Y., Kang, S., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>, Shiba, K., Choi, T. S., Moon, S., Litvin, S., Lee, K.,  &amp; Lee, J.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014', 'https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992', event);\">\n    Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO$_{\\textrm{2}}$.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Journal of Marine Systems</i>, 137: 67–73. September 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\"\n     onclick=\"log_download('sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014', 'https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO$_{\\textrm{2}}$ [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.jmarsys.2014.04.013\"\n     onclick=\"log_download('sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014', 'http://doi.org/10.1016/j.jmarsys.2014.04.013', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{sung_species_2014,\n\ttitle = {Species and gamete-specific fertilization success of two sea urchins under near future levels of {pCO}$_{\\textrm{2}}$},\n\tvolume = {137},\n\tissn = {09247963},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992},\n\tdoi = {10.1016/j.jmarsys.2014.04.013},\n\tlanguage = {en},\n\turldate = {2021-07-27},\n\tjournal = {Journal of Marine Systems},\n\tauthor = {Sung, Chan-Gyung and Kim, Tae Won and Park, Young-Gyu and Kang, Seong-Gil and Inaba, Kazuo and Shiba, Kogiku and Choi, Tae Seob and Moon, Seong-Dae and Litvin, Steve and Lee, Kyu-Tae and Lee, Jung-Suk},\n\tmonth = sep,\n\tyear = {2014},\n\tpages = {67--73},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Yokoe, M., Sano, M., Shibata, H., Shibata, D., Takayama-Watanabe, E., <a class=\"bibbase author link\" href=\"https://www.shimoda.tsukuba.ac.jp/staff_inaba.html\">Inaba, K.</a>,  &amp; Watanabe, A.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/9/15210\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014', 'http://www.mdpi.com/1422-0067/15/9/15210', event);\">\n    Sperm Proteases that May Be Involved in the Initiation of Sperm Motility in the Newt, <i>Cynops pyrrhogaster</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>International Journal of Molecular Sciences</i>, 15(9): 15210–15224. August 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/9/15210\"\n     onclick=\"log_download('yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014', 'http://www.mdpi.com/1422-0067/15/9/15210', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Sperm Proteases that May Be Involved in the Initiation of Sperm Motility in the Newt, &lt;i&gt;Cynops pyrrhogaster&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/ijms150915210\"\n     onclick=\"log_download('yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014', 'http://doi.org/10.3390/ijms150915210', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{yokoe_sperm_2014,\n\ttitle = {Sperm {Proteases} that {May} {Be} {Involved} in the {Initiation} of {Sperm} {Motility} in the {Newt}, \\textit{{Cynops} pyrrhogaster}},\n\tvolume = {15},\n\tissn = {1422-0067},\n\turl = {http://www.mdpi.com/1422-0067/15/9/15210},\n\tdoi = {10.3390/ijms150915210},\n\tlanguage = {en},\n\tnumber = {9},\n\turldate = {2021-07-27},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Yokoe, Misato and Sano, Makoto and Shibata, Honami and Shibata, Daisuke and Takayama-Watanabe, Eriko and Inaba, Kazuo and Watanabe, Akihiko},\n\tmonth = aug,\n\tyear = {2014},\n\tpages = {15210--15224},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n\n\n\n  </div>\n\n\n  <!-- Modal -->\n\n  <!-- <iframe id=\"bibbase_network_frame\" -->\n  <!--         src=\"//bibbase.org/network/control\" -->\n  <!--         style=\"display: none;\"> -->\n  <!-- </iframe> -->\n\n</div>\n"}; document.write(bibbase_data.data);